Trypanosoma brucei and Trypanosoma cruzi DNA Mismatch Repair Proteins Act Differently in the Response to DNA Damage Caused by Oxidative Stress.
作者： Viviane Grazielle-Silva , Tehseen Fatima Zeb , Richard Burchmore , Carlos Renato Machado , Richard McCulloch
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摘要: MSH2, associated with MSH3 or MSH6, is a central component of the eukaryotic DNA Mismatch Repair (MMR) pathway responsible for recognition and correction base mismatches that occur during replication recombination. Previous studies have shown MSH2 plays an additional repair role in response to oxidative damage Trypanosoma cruzi brucei. By performing co-immunoprecipitation followed by mass spectrometry parasites expressing tagged proteins, we confirmed parasites’ forms complexes MSH6. To investigate involvement these two other MMR components stress response, generated knockout mutants MSH6 T. brucei bloodstream epimastigotes. Differently from phenotype observed epimastigotes, loss one alleles msh6 resulted increased susceptibility H2O2 exposure, besides impaired MMR. In contrast, msh3 null displayed tolerance MNNG treatment, indicating affected, but no difference treatment when compared wild type cells. Taken together, our results suggest that, while are involved addition their as MMR, deals operates differently
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sci-hub.se 参考文章(50)
Zhongdao Li, Alexander H. Pearlman, Peggy Hsieh, DNA mismatch repair and the DNA damage response. DNA Repair. ,vol. 38, pp. 94- 101 ,(2016) , 10.1016/J.DNAREP.2015.11.019
Viviane Grazielle-Silva, Tehseen Fatima Zeb, Jason Bolderson, Priscila C. Campos, Julia B. Miranda, Ceres L. Alves, Carlos R. Machado, Richard McCulloch, Santuza M. R. Teixeira, Distinct Phenotypes Caused by Mutation of MSH2 in Trypanosome Insect and Mammalian Life Cycle Forms Are Associated with Parasite Adaptation to Oxidative Stress. PLOS Neglected Tropical Diseases. ,vol. 9, ,(2015) , 10.1371/JOURNAL.PNTD.0003870
David Horn, Sam Alsford, Single-locus targeting constructs for reliable regulated RNAi and transgene expression in Trypanosoma brucei. Molecular and Biochemical Parasitology. ,vol. 161, pp. 76- 79 ,(2008) , 10.1016/J.MOLBIOPARA.2008.05.006
Gabriela Schumann Burkard, Pascal Jutzi, Isabel Roditi, Genome-wide RNAi screens in bloodstream form trypanosomes identify drug transporters. Molecular and Biochemical Parasitology. ,vol. 175, pp. 91- 94 ,(2011) , 10.1016/J.MOLBIOPARA.2010.09.002
B Räz, M Iten, Y Grether-Bühler, R Kaminsky, R Brun, The Alamar Blue® assay to determine drug sensitivity of African trypanosomes (T.b. rhodesiense and T.b. gambiense) in vitro Acta Tropica. ,vol. 68, pp. 139- 147 ,(1997) , 10.1016/S0001-706X(97)00079-X
Niels de Wind, Marleen Dekker, Anton Berns, Miroslav Radman, Hein te Riele, Inactivation of the mouse Msh2 gene results in mismatch repair deficiency, methylation tolerance, hyperrecombination, and predisposition to cancer Cell. ,vol. 82, pp. 321- 330 ,(1995) , 10.1016/0092-8674(95)90319-4
K. R. Matthews, The developmental cell biology of Trypanosoma brucei. Journal of Cell Science. ,vol. 118, pp. 283- 290 ,(2005) , 10.1242/JCS.01649
Danielle Gomes Passos-Silva, Matheus Andrade Rajão, Pedro Henrique Nascimento de Aguiar, João Pedro Vieira-da-Rocha, Carlos Renato Machado, Carolina Furtado, Overview of DNA Repair in Trypanosoma cruzi, Trypanosoma brucei, and Leishmania major Journal of Nucleic Acids. ,vol. 2010, pp. 840768- 840768 ,(2010) , 10.4061/2010/840768
Gabriela Burkard, Cristina M. Fragoso, Isabel Roditi, Highly efficient stable transformation of bloodstream forms of Trypanosoma brucei Molecular and Biochemical Parasitology. ,vol. 153, pp. 220- 223 ,(2007) , 10.1016/J.MOLBIOPARA.2007.02.008
J. R. STEVENS, H. A. NOYES, G. A. DOVER, W. C. GIBSON, The ancient and divergent origins of the human pathogenic trypanosomes, Trypanosoma brucei and T. cruzi. Parasitology. ,vol. 118, pp. 107- 116 ,(1999) , 10.1017/S0031182098003473