Differentiation state of skin fibroblast cultures versus risk of subcutaneous fibrosis after radiotherapy

摘要: Abstract Background and purpose : There is increasing evidence for patient-to-patient variation in the response of normal tissue to radiotherapy. Recently, it has been suggested that accumulation functional fibrocytes may be a key step development radiation-induced fibrosis. Therefore, we have examined possible relationship between differentiation state untreated fibroblasts risk subcutaneous fibrosis individual patients. Materials methods We used skin fibroblast cultures isolated from eight postmastectomy radiotherapy patients whose clinical radiosensitivity was assessed by mean excess Different types potentially mitotic progenitor (MF) postmitotic (PMF) terminal lineage, MFI→MFII→MFIII→PMF, were scored morphologically clonal culture. Progression quantified ratio L/E colony-forming late (MFIII MFII) early (MFI progenitors. Results observed correlation ( r S =0.743, P =0.03), indicating an approximately 10-fold increase with This paralleled decreasing trend absolute numbers types. By contrast, there no significant plating efficiency Conclusions The data suggest increases progression fibroblasts, co-factor If this hypothesis validated, provides rationale novel predictive test identify increased