Imaging adipose tissue browning using the TSPO-18kDa tracer [18F]FEPPA

摘要: Abstract Objectives The browning of white adipose tissue (WAT) into beige has been proposed as a strategy to enhance energy expenditure combat the growing epidemic obesity. Research strategies are hampered by lack sensitive, translatable, imaging tools capable detecting fat mass non-invasively. [18F]FDG is able detect activated but provides little information on unstimulated mass. We have assessed use [18F]FEPPA, tracer for TSPO-18KDa found outer mitochondrial membrane, an alternative agent brown (BAT) and fat. Methods Female Balb/c mice (n = 5) were treated 7 days with β3 adrenergic agonist CL-316,243 induce inguinal WAT (beige fat). Animals imaged longitudinally [18F]FEPPA uptake in interscapular BAT assessed. was confirmed using H&E immunohistochemical detection UCP-1 TSPO. Results Repeated dosing β3-adrenergic caused significant increase both associated increased metabolic activity adipocytes respectively. likewise showed no due stimulation over same time course. Furthermore, unaffected pharmacological blockade, indicating that expression mitochondria independent activation. Conclusion These data show can newly formed under non-stimulated, thermoneutral conditions after linked opposed