Prediction of the immunogenic potential of frameshift-mutated antigens in microsatellite instable cancer.

摘要: Microsatellite instable (MSI) cancers express frameshift-mutated antigens, the C-terminal polypeptides of which are foreign to immune system. Consequently, these antigens constitute a unique pool tumor-specific that can be exploited for patient diagnosis and selective, immune-mediated targeting cancers. However, other than their sequence, very little is known about characteristics majority proteins. We therefore developed methodology predicting immunogenic behavior based on gene-expression system, in each proteins was fused short polypeptide comprising two epitopes readily detected by T-cells antibodies, respectively. In this manner, accumulation processing peptides derived thereof into MHC monitored systematically. The accumulate cells they synthesized, primary interest cancer immunotherapy, because peptide presented dendritic addition tumor themselves. As result, best targets coordinated response both CD8+ CD4+ T-cells, increases likelihood tumor-induced immunity would detectable against patients, as well potential value components anticancer vaccines. Our data indicate that, 15 examined our study, 4 (TGFβR2-1, MARCKS-1, MARCKS-2 CDX2-2) interest, additional (TAF1B-1, PCNXL2-2, TCF7L2-2 Baxα+1) moderate further immunological research. © 2008 Wiley-Liss, Inc.