Boundary analysis in sedimentation transport experiments: A procedure for obtaining sedimentation coefficient distributions using the time derivative of the concentration profile

摘要: Abstract A procedure is described for computing sedimentation coefficient distributions from the time derivative of velocity concentration profile. Use derivative, ( ∂c ∂t ) r , instead radial ∂r t desirable because it independent time-invariant contributions to optical baseline. Slowly varying baseline changes also are significantly reduced. An apparent distribution (i.e., uncorrected effects diffusion), g ∗ (s) can be calculated as = corr 1 c o ω 2 1n( m where s coefficient, angular rotor, c0 initial concentration, radius, rm radius meniscus, and time. iterative presented by taking into account contribution plateau region give . Values obtained this way identical those within roundoff error computations. results in a significant increase (>10-fold) signal-to-noise ratio data both uv photoelectric scanner Rayleigh systems analytical ultracentrifuge. The use compute purposes boundary analysis exemplified with an antigen-antibody system.