Real-Time Visualization and Quantitation of Vascular Permeability In Vivo: Implications for Drug Delivery

摘要: The leaky, heterogeneous vasculature of human tumors prevents the even distribution systemic drugs within cancer tissues. However, techniques for studying vascular delivery systems in vivo often require complex mammalian models and time-consuming, surgical protocols. developing chicken embryo is a well-established model that easily accessible tumor imaging. To assess this analysis permeability, were grown on chorioallantoic membrane (CAM), thin which overlays growing chick embryo. real-time movement small fluorescent dextrans through surrounding tissues used to measure leak xenografts. Dextran extravasation sites was selectively enhanced an interleukin-2 (IL-2) peptide fragment or endothelial growth factor (VEGF). VEGF treatment increased core relative normal tissue doxorubicin uptake This new system visualizes permeability changes suggests may be manipulated improve chemotherapeutic targeting tumors.