Density- and serum-dependent regulation of the Reck tumor suppressor in mouse embryo fibroblasts.

摘要: Reck is a membrane-anchored glycoprotein identified as transformation suppressor. Accumulating evidence indicates that negatively regulates wide spectrum of matrix metalloproteinases and commonly down-regulated in variety malignant solid tumors. Physiological cues regulate expression, however, remained unknown. In this study, we found expression was up-regulated at high cell density, low serum, or after treatment with some kinase inhibitors, such PP2 (Src inhibitor), LY294002 (PI3-kinase PF573228 (FAK mouse embryo fibroblasts. Curve fitting indicated the levels protein mRNA are quadratic density. Other factors, including extracellular components (type I collagen fibronectin), their oncogenic targets (v-Src PIK3CA mutants), modify shape curve. Comparison these modifications implicated Src down-regulation under sparse conditions, PI3-kinase serum-induced down-regulation, FAK Fibronectin type Reck, supporting role integrin-FAK signaling Our study has revealed multiple pathways impinging on cultured fibroblasts sets foundation for future studies to find effective inducers potential value cancer therapy.