Keratinization-associated miR-7 and miR-21 Regulate Tumor Suppressor Reversion-inducing Cysteine-rich Protein with Kazal Motifs (RECK) in Oral Cancer

作者: Hyun Min Jung , Brittany L. Phillips , Rushi S. Patel , Donald M. Cohen , Andrew Jakymiw

DOI: 10.1074/JBC.M112.366518

关键词:

摘要: MicroRNAs (miRNAs) are small non-coding RNAs that posttranscriptionally regulate gene expression during many biological processes. Recently, the aberrant expressions of miRNAs have become a major focus in cancer research. The purpose this study was to identify deregulated oral and further on specific were related patient survival. Here, we report miRNA profiling provided more precise information when squamous cell carcinomas subcategorized basis clinicopathological parameters (tumor primary site, histological subtype, tumor stage, HPV16 status). An innovative radar chart analysis method developed depict subcategories cancers taking into consideration patterns multiple combined with parameters. Keratinization tumors high miR-21 factors poor prognosis patients. Interestingly, majority keratinized expressed levels miR-21. Further investigations demonstrated regulation suppressor reversion-inducing cysteine-rich protein kazal motifs (RECK) by two keratinization-associated miRNAs, miR-7 Transfection miR-21-mimics reduced RECK through direct miRNA-mediated regulation, these inversely correlated CAL 27 orthotopic xenograft tumors. Furthermore, similar inverse correlation cells treated vitro different external stimuli such as trypsinization, density, serum concentration. Taken together, our data show keratinization is associated patients mediate deregulation which may contribute aggressiveness

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