Up-regulation of D1A dopamine receptor gene transcription by estrogen.
作者： Sang-Hyeon Lee , M.Maral Mouradian
DOI: 10.1016/S0303-7207(99)00133-1
关键词:
摘要: Abstract Estrogen exerts complex physiologic effects on brain functions which could partly be mediated through modulation of the dopaminergic system. Transcription control human D1A dopamine receptor gene by estrogenic stimulation was studied in expressing neuroblastoma cell line SK-N-MC. Transient co-transfection promoter-CAT constructs along with expression vectors for steroid hormone receptors indicated that estrogen, but not progesterone or glucocorticoid, up-regulate transcription this about 1.7-fold. Serial 5′ deletion mutants upstream region localized estrogen responsive segment between nucleotides −1472 and −1342 relative to initiator methionine. This contains a half palindrome (TGACC) consensus element (ERE). Additional experiments revealed specifically activate promoter downstream located intron gene. Consistent transient experiments, 17β-estradiol treatment SK-N-MC cells transfected an vector resulted approximately 20% increase steady-state levels long transcripts derived from short originating promoter. These observations demonstrate molecular basis induced up-regulation provide mechanism central hormone.
elsevier.com
sci-hub.se 参考文章(32)
M. Ponglikitmongkol, J. H. White, P. Chambon, Synergistic activation of transcription by the human estrogen receptor bound to tandem responsive elements. The EMBO Journal. ,vol. 9, pp. 2221- 2231 ,(1990) , 10.1002/J.1460-2075.1990.TB07392.X
M. E. Meyer, A. Pornon, J. W. Ji, M. T. Bocquel, P. Chambon, H. Gronemeyer, Agonistic and antagonistic activities of RU486 on the functions of the human progesterone receptor. The EMBO Journal. ,vol. 9, pp. 3923- 3932 ,(1990) , 10.1002/J.1460-2075.1990.TB07613.X
N.G. Miyamoto, V. Moncollin, J.M. Egly, P. Chambon, Specific interaction between a transcription factor and the upstream element of the adenovirus-2 major late promoter. The EMBO Journal. ,vol. 4, pp. 3563- 3570 ,(1985) , 10.1002/J.1460-2075.1985.TB04118.X
L. Tora, M. P. Gaub, S. Mader, A. Dierich, M. Bellard, P. Chambon, Cell-specific activity of a GGTCA half-palindromic oestrogen-responsive element in the chicken ovalbumin gene promoter. The EMBO Journal. ,vol. 7, pp. 3771- 3778 ,(1988) , 10.1002/J.1460-2075.1988.TB03261.X
Arun K. Rishi, Shioko Kimura, Joseph A. Fontana, Richard G. Baumann, M. Saeed Sheikh, Nasir Bashirelahi, Xiao-Su Li, Zhi-Ming Shao, Estradiol Regulation of the Human Retinoic Acid Receptor α Gene in Human Breast Carcinoma Cells Is Mediated via an Imperfect Half-Palindromic Estrogen Response Element and Sp1 Motifs Cancer Research. ,vol. 55, pp. 4999- 5006 ,(1995)
S Richard, H H Zingg, The human oxytocin gene promoter is regulated by estrogens. Journal of Biological Chemistry. ,vol. 265, pp. 6098- 6103 ,(1990) , 10.1016/S0021-9258(19)39297-X
M.T. Minowa, T Minowa, M.M. Mouradian, Activator region analysis of the human D1A dopamine receptor gene. Journal of Biological Chemistry. ,vol. 268, pp. 23544- 23551 ,(1993) , 10.1016/S0021-9258(19)49497-0
F.L. Graham, A.J. van der Eb, A new technique for the assay of infectivity of human adenovirus 5 DNA Virology. ,vol. 52, pp. 456- 467 ,(1973) , 10.1016/0042-6822(73)90341-3
N. Giladi, S. Honigman, Hormones and Parkinson's disease. Neurology. ,vol. 45, pp. 1028- 1028 ,(1995) , 10.1212/WNL.45.5.1028-A
R. Hruska, E. Silbergeld, Increased dopamine receptor sensitivity after estrogen treatment using the rat rotation model Science. ,vol. 208, pp. 1466- 1468 ,(1980) , 10.1126/SCIENCE.7189902