Transfer of cytoplasmic and nuclear proteins between cells in culture.
作者： G.M. Kolodny
DOI: 10.1016/0022-2836(73)90438-5
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摘要: Abstract Evidence is presented for transfer of proteins between cells in culture, using techniques which previously have shown RNA and the lack DNA culture. These involved making donor heavier than recipient by having them ingest tantalum particles. After coculture two cell types were separated centri- fugation on Ficoll gradients analyzed radioactively labeled that may passed from prelabeled cells. also provided evidence passage to nuclei. Examination nuclear revealed histones transferred intercellularly a greater extent other proteins. The histone subfractions nuclei studied acrylamide gel electrophoresis. No major differences found proportion each subfraction was
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