Anti-inflammatory effect of hexane fraction from Myagropsis myagroides ethanolic extract in lipopolysaccharide-stimulated BV-2 microglial cells.

摘要: Objectives Microglial activation has been implicated in neurological disorders for its inflammatory and neurotrophic effects. We investigated the anti-inflammatory effect of hexane fraction from Myagropsis myagroides (Mertens ex Turner) Fensholt ethanolic extract underlying molecular mechanism lipopolysaccharide-stimulated microglia. Methods Various solvent fractions prepared M. myagroides were analysed total phenolic content, 2,2-diphenyl-1-picrylhydrazyl radical-scavenging activity inhibitory on nitric oxide (NO) production activated BV-2 microglia. measured prostaglandin E2 (PGE2) pro-inflammatory cytokine levels by enzyme-linked immunosorbent assay. Expression enzymes was Western blot. Nuclear translocation nuclear factor-kappaB (NF-κB) determined immunofluorescence reporter gene assay, respectively. Key findings Among fractions, (MMH), rich fatty acid, showed highest NO generation. Pretreatment with MMH decreased mRNA protein inducible synthase cyclooxygenase-2, resulting a decrease PGE2 LPS-stimulated cells. Furthermore, inhibited cytokines at their transcriptional level via inactivation NF-κB. extracellular signal-regulated kinase c-Jun N-terminal kinase. Conclusions These results indicate that strong microglia, suggesting can be used as therapeutic agent against neuroinflammatory diseases.