[6] Neoglycoprotein-liposome and lectin-liposome conjugates as tools for carbohydrate recognition research

摘要: Publisher Summary This chapter describes the procedures for preparation and characterization of one type versatile stable liposomes as well covalent conjugation neoglycoproteins lectins to liposomes, conjugates. To analyze homogeneity stability size distribution is an important characteristic among several physical properties. Gel-permeation chromatography (GPC), electron microscopy (EM), dynamic light scattering (DLS) can be applied liposomes. The discusses procedure GPC analysis. A result compared with analyses by EM DLS. purity neoglycoprotein-coupled or lectin-coupled analysis routinely carried out using high-performance liquid (HPLC) system. yield quality protein lipid compositions in each are determined, coupled protein/total ratios calculated. content neoglycoprotein-liposome lectin-liposome conjugates estimated either employing modified Lowry method presence 1% sodium dodecyl sulfate, a commercial micro-bovine serum albumin (BSA) assay kit sulfate.