Studies on carbohydrate-binding proteins using liposome-based systems—I. Preparation of neoglycoprotein-conjugated liposomes and the feasibility of their use as drug-targeting devices

摘要: Abstract 1. Five types of neoglycoprotein-coupled liposomes were prepared in order to investigate their potential utility as new drug-targeting devices which exploit cellular functions carbohydrate-binding proteins. 2. These preparations shown be stable at 37°C for 24 hr and 7°C over 4 months. 3. An inhibition assay an vitro system using human adenocarcinoma cells indicated the high affinity binding neoglycoprotein-conjugated liposomes. The inhibitory potency correlated with both type amount immobilized neoglycoproteins on 4. A tissue distribution vivo Ehrlich solid tumor-bearing mice showed feasibility application [ 125 I]neoglycoprotein-conjugated devices, based carbohydrate-protein interactions.