Separation and identification of cytokinins using combined capillary liquid chromatography/mass spectrometry
作者： Nadine Imbault , Thomas Moritz , Ove Nilsson , Hao-Jie Chen , Marie Bollmark
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摘要: Non-derivatized cytokinins were analysed by liquid chromatography/mass spectrometry (LC/MS). The effluent from a capillary reversed-phase high-performance chromatography column was introduced into the ion source of double-focusing mass spectrometer via frit interface, and ions generated fast atom bombardment (FAB) with 1% glycerol in mobile phase acting as matrix. Positive FAB spectra obtained for base, riboside, ribotide glucoside forms cytokinins. found to provide useful information identification structural elucidation LC/MS system used identify iso-pentenyladenosine purified extract Norway spruce (Picea abies) needles. Quantitative analysis using (2H6)isopentenyladenosine internal standard indicated levels 1.2 ng g−1 fresh weight.
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