Studies on transmission of hepatitis A virus to squirrel monkeys

摘要: Non-human primates have been playing an essential role in the study of hepatitis A virus (HAV) biology, pathogenesis and for testing candidate HAV vaccines. This was to determine suitability squirrel monkeys (Saimiri sciureus) as animal model infection. Animals were inoculated, either intragastrically or intravenously, with a Brazilian isolate (HAF-203). Alanine aminotransferase (ALT) anti-HAV antibodies (IgM total) monitored. Feces daily collected antigen RNA detection. Samples liver tissue obtained by biopsy before inoculation at peak ALT levels and/or when developed, necropsy morphological examination. Monkeys inoculated intravenous route rapidly developed significant elevations serum ALT, antibodies, histologic changes, while only evidence infection animals seroconversion. Moreover, excreted very low detectable few fecal samples after amplification RT-PCR, different from humans other non-human primate species that eliminate large quantities during late incubation period. The unusual onset experimentally infected represent important obstacle its use this viral However, they can valuable tool obtention hyperimmune sera HAV, view high titer (105) 24 days single inoculation.