Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen
作者： Claudia Lamarca Vitral , Ana Maria Coimbra Gaspar , Clara Fumiko Tachibana Yoshida
DOI: 10.1590/S0037-86821991000200003
关键词:
摘要: Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HA VHRP conjugate; second (COMP-2), and conjugate competed for epitopes previously adsorbed to V wells. Both procedures used tetramethylbenzidine (TMB) as a substrate. tests shown be reproducible suitable routine diagnosis research purposes.
sci-hub.se 参考文章(24)
G. G. Frösner, F. Deinhardt, R. Scheid, V. Gauss-Müller, N. Holmes, V. Messelberger, G. Siegl, J. J. Alexander, Propagation of human hepatitis A virus in a hepatoma cell line. Infection. ,vol. 7, pp. 303- 305 ,(1979) , 10.1007/BF01642154
Robert H. Purcell, Doris C. Wong, Harvey J. Alter, Paul V. Holland, Microtiter Solid-Phase Radioimmunoassay for Hepatitis B Antigen Applied Microbiology. ,vol. 26, pp. 478- 484 ,(1973) , 10.1128/AM.26.4.478-484.1973
A G Coulepis, M F Veale, A MacGregor, M Kornitschuk, I D Gust, Detection of hepatitis A virus and antibody by solid-phase radioimmunoassay and enzyme-linked immunosorbent assay with monoclonal antibodies. Journal of Clinical Microbiology. ,vol. 22, pp. 119- 124 ,(1985) , 10.1128/JCM.22.1.119-124.1985
Yasuo Moritsugu, Jules L. Dienstag, John A. Routenberg, Robert H. Purcell, Doris C. Wong, Joseph D. Boggs, A Microtiter Solid-Phase Radioimmunoassay for Hepatitis a Antigen and Antibody Journal of Immunology. ,vol. 116, pp. 349- 356 ,(1976)
C. Girardet, R. Peitrequin, P. C. Frei, Serodiagnosis of viral hepatitis A: Rise in antibody titre and evaluation of three methods for detecting early and late antibodies Experientia. ,vol. 39, pp. 902- 904 ,(1983) , 10.1007/BF01990429
Hideo Kojima, Takao Shibayama, Akira Sato, Shiro Suzuki, Fumihiro Ichida, Chuya Hamada, Propagation of human hepatitis A virus in conventional cell lines Journal of Medical Virology. ,vol. 7, pp. 273- 286 ,(1981) , 10.1002/JMV.1890070404
E. S. Bos, A. A. van der Doelen, N. van Rooy, A. H.W.M. Schuurs, 3,3',5,5' - Tetramethylbenzidine as an Ames test negative chromogen for horse-radish peroxidase in enzyme-immunoassay. Journal of Immunoassay. ,vol. 2, pp. 187- 204 ,(1981) , 10.1080/15321818108056977
Roslyn E. Wallace, Paul J. Vasington, John C. Petricciani, Hope E. Hopps, Douglas E. Lorenz, Zdenek Kadanka, Development and characterization of cell lines from subhuman primates. In Vitro Cellular & Developmental Biology – Plant. ,vol. 8, pp. 333- 341 ,(1973) , 10.1007/BF02619057
R. S. Tedder, R. Bridget Lord, P. P. Mortimer, Detection of antibody to varicella‐zoster virus by competitive and IgM‐antibody capture immunoassay Journal of Medical Virology. ,vol. 8, pp. 89- 101 ,(1981) , 10.1002/JMV.1890080203
P. J. Provost, O. L. Ittensohn, V. M. Villarejos, M. R. Hilleman, A specific complement-fixation test for human hepatitis a employing CR326 virus antigen. Diagnosis and epidemiology. Experimental Biology and Medicine. ,vol. 148, pp. 962- 969 ,(1975) , 10.3181/00379727-148-38669