Propagation of human hepatitis A virus in conventional cell lines
作者： Hideo Kojima , Takao Shibayama , Akira Sato , Shiro Suzuki , Fumihiro Ichida
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摘要: Fecal extracts of hepatitis A (HA) patients were selected for the presence virus (HAV) by radioimmunoassay (RIA) and immune electron microscopy (IEM). When FL Vero cells inoculated with fecal containing HAV, development antigen (HAAg) was evident in cytoplasm two cell lines indirect immunofluorescence (IF) test. The detectable 12 hr postinoculation (pi), reached a plateau within days pi. cultures specimen HAV harvested passaged four times. During passages, efficient production HAAg confirmed infected three different serological tests: IF test, RIA using fixed cells, sandwich method. At second fourth particles recovered abundance from IEM. Throughout these experiments, no cytopathic effect (CPE) discernible cultures.
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