Adaptation of hepatitis A virus to high titre growth in diploid and permanent cell cultures.

摘要: A hepatitis virus isolate originally obtained from the feces of a clinically ill patient and passaged in diploid human embryonic kidney lung cells was adapted to grow MRC-5, Cercopithecus aethiops muscle Vero cells. Three different adaptation methods were applied. Either method proved be suitable finally give high titres cell-bound as well cell-free supernatant infected cultures during 10 15 passages. An easily performable immunoperoxidase staining used for titration microtitre plates. Cytopathogenic changes MRC-5 cell with fully are described.