Epidermal growth factor transcription, translation, and signal transduction by rat type II pneumocytes in culture.

作者: Lasse Raaberg , Ebba Nexø , Sue Buckley , Wen Luo , Malcolm L. Snead

DOI: 10.1165/AJRCMB/6.1.44

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摘要: Epidermal growth factor (EGF) is known to induce fetal lung maturation and its receptor present in the lungs of several species. Recently, EGF has been immunolocalized type II pneumocytes rat lung. We postulated that synthesized that, because position-restricted distribution within alveolus, might act as an autocrine regulator pneumocyte function. Herein, we have tested hypothesis using adult primary culture. In situ hybridization, oligonucleotide probe corresponding amino acid residues 1070 1081 mouse precursor, demonstrated presence precursor mRNA. Upon S-200 Sephacryl gel chromatography extracts, EGF-reactive protein eluted a high-molecular-weight form (> 100 kD). immunoreactivity was localized periphery groups 10 15 cells The bound [125I]EGF specific manner, indic...

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