Wolbachia Infections Are Virulent and Inhibit the Human Malaria Parasite Plasmodium Falciparum in Anopheles Gambiae

摘要: Endosymbiotic Wolbachia bacteria are potent modulators of pathogen infection and transmission in multiple naturally artificially infected insect species, including important vectors human pathogens. Anopheles mosquitoes uninfected with Wolbachia, stable artificial infections have not yet succeeded this genus. Recent techniques enabled establishment somatic Anopheles. Here, we characterize two diverse strains (wMelPop wAlbB) gambiae, the major vector malaria. After infection, wMelPop disseminates widely mosquito, infecting fat body, head, sensory organs other tissues but is notably absent from midgut ovaries. initially induces mosquito immune system, coincident initial clearing then suppresses expression genes, replication mosquito. Both wAlbB significantly inhibit Plasmodium falciparum oocyst levels midgut. Although virulent non-bloodfed mosquitoes, exhibits a novel phenotype extremely for approximately 12–24 hours post-bloodmeal, after which surviving exhibit similar mortality trajectories to control mosquitoes. The data suggest that if transinfections act manner infections, could potentially be used as part strategy transmit