Molecular detection and characterisation of fungal heat shock protein 60.

摘要: Summary Heat shock proteins (Hsp) are highly conserved molecules, which both constitutively expressed and up-regulated in response to various stress conditions. In particular, fungal Hsp60 can act as immunodominant antigens facilitate powerful immunological properties. A possible cellular heat was investigated eight fungi (Aspergillus fumigatus, Aspergillus terreus, Penicillium chrysogenum, Cladosporium cladosporioides, Scedosporium apiospermum, Trichophyton mentagrophytes, Candida albicans Saccharomyces cerevisiae). Fully automated RNA extraction followed by quantitative real-time RT-PCR targeting fungus-specific mRNA sequencing of the amplicon. Levels temperature-dependent gene expression were evaluated rates similarity identity compared. While all samples tested, a induction not shown C. cladosporioides. 80-amino acid fragment from hypothetical protein, 66% amino acids identical, 20% showed 8% semi-conserved substitution. Our findings should contribute better understanding host–pathogen relationship suggest that under temperature-related conditions might an immunogenic trigger orchestrating fungi-related diseases.