Assignment of 1H nuclear magnetic resonance visible polyunsaturated fatty acids in BT4C gliomas undergoing ganciclovir-thymidine kinase gene therapy-induced programmed cell death.

摘要: Polyunsaturated fatty acids (PUFAs), as detected by 1H nuclear magnetic resonance (NMR) spectroscopy, accumulate into BT4C glioma during ganciclovir-thymidine kinase gene therapy-induced programmed cell death (PCD). In this study, we have quantified the NMR visible lipids in vivo and characterized their biophysical biochemical nature these tumors PCD both ex vitro . Concentrations of NMR-detectable PUFAs increased 3-fold with pattern recognition identifying C H = CH CHC 2CH most significant monitoring dynamics PCD. The increase was equivalent to 70% that CH2C 2CH2-saturated lipid peak at 1.3 ppm. Ex tumor samples, obtained from situ funnel frozen tumors, showed very similar macromolecular peaks, studied using high-resolution magic angle spinning 14.1 T, those 4.7 T. Line widths peaks were not influenced spin rate within range 1–9 kHz or temperature between 277 293 K, showing high degree detection These results additionally corroborate idea cytoplasmic vesicles are source signals. Two-dimensional extracts PUFA signals same chemical compounds consist largely 18:1 18:2 lipids. Furthermore, it is suggested arise constituent breakdown products forming dying cells.