Assessment of a Novel VEGF Targeted Agent Using Patient-Derived Tumor Tissue Xenograft Models of Colon Carcinoma with Lymphatic and Hepatic Metastases

摘要: The lack of appropriate tumor models primary tumors and corresponding metastases that can reliably predict for response to anticancer agents remains a major deficiency in the clinical practice cancer therapy. It was aim our study establish patient-derived tissue (PDTT) xenograft colon carcinoma with lymphatic hepatic useful testing novel molecularly targeted agents. PDTT carcinoma, were used create models. Hematoxylin eosin staining, immunohistochemical genome-wide gene expression analysis, pyrosequencing, qRT-PCR, western blotting determine biological stability xenografts during serial transplantation compared original tissues. Early passages revealed high degree similarity samples regard histology, immunohistochemistry, genes expression, mutation status as well mRNA expression. After we have ascertained these retained similar histopathological features molecular signatures tumors, drug sensitivities VEGF agent, FP3 evaluated. In this study, metastasis been successfully established. They provide