Development and characterization of a bladder cancer xenograft model using patient-derived tumor tissue.
作者： Bumsoo Park , Byong Chang Jeong , Yoon-La Choi , Ghee Young Kwon , Joung Eun Lim
DOI: 10.1111/CAS.12123
关键词:
摘要: Most of the cancer xenograft models are derived from tumor cell lines, but they do not sufficiently represent clinical characteristics. Our objective was to develop bladder human tissue and characterize them molecularly as well histologically. A total 65 tissues were transplanted immunodeficient mice. Passagable six cases with clinico-pathologically heterogeneous selected their collected (012T, 025T, 033T, 043T, 048T, 052T). Xenografts removed processed for following analyses: (i) histologic examination, (ii) short tandem repeat (STR) genotyping, (iii) mutational analysis, (iv) array-based comparative genomic hybridization (array-CGH). The original (P 0) xenografts passage 2 or higher (≥P2) analyzed compared. As a result, hematoxylin eosin staining revealed same architecture degree differentiation in primary tumors all cases. Xenograft 043T_P2 048T_P2 had completely identical STR profiles samples loci. other nearly profiles. On four out mutations TP53, HRAS, BRAF, CTNNB1. Array-CGH analysis that alterations similar samples. In conclusion, our model patient is expected be useful studying heterogeneity populations evaluating new treatments.
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