Effect of regulating cholesterol biosynthesis on breath isoprene excretion in men

摘要: Isoprene is a normal constituent of human breath and may be derived from the cholesterol synthetic pathway. Acute chronic lovastatin cholesterol-supplemented diet were used to determine whether mechanistic link exists between isoprene biosynthesisin vivo in humans. The acute effects lovastatin, competitive inhibitor rate-limiting step biosynthesis, on excretion was determined by administering single 20, 40 or 80 mg dose this drug five healthy male subjects at 8 p.m. measuring their levels every 4 h for one 24 cycle before after treatment. When compared baseline cycle, all three doses significantly reduced 6 10 post-drug Chronic therapy (40 b.i.d. wk) a.m. (time maximum value) 27 ± 9% (SEM) synthesis measured freshly isolated mononuclear leukocytes (ML) 12 6%. A (1070 mg, total) ingested wk ML sterol 16 5 19 4%, respectively. parallel decreases caused these pharmacologic dietary means suggest that