Use of synchrotron medical microbeam irradiation to investigate radiation-induced bystander and abscopal effects in vivo.

摘要: The question of whether bystander and abscopal effects are the same is unclear. Our experimental system enables us to address this by allowing irradiated organisms partner with unexposed individuals. Organs from both animals appropriate sham scatter dose controls tested for expression several endpoints such as calcium flux, role 5HT, reporter assay cell death proteomic profile. results show that membrane related functions 5HT critical true effect expression. original inter-animal experiments used fish species whole body low doses X-rays, which prevented addressing question. Data much more relevant in radiotherapy now available rats received high local irradiation implanted right brain glioma. data were generated using quasi-parallel microbeams at biomedical beamline European Synchrotron Radiation Facility Grenoble France. This means we can directly compare “true” a rodent tumour model. Analysis hemisphere, left urinary bladder their unirradiated partners strongly suggests (in animals) not animal). Furthermore, presence alters magnitude tissues animal did contain tumours, meaning type signal was different.