Orexins Suppress Catecholamine Synthesis and Secretion in Cultured PC12 Cells
作者： Toru Nanmoku , Kazumasa Isobe , Takeshi Sakurai , Akihiro Yamanaka , Kazuhiro Takekoshi
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摘要: New orexigenic peptides called orexin-A and -B have recently been described in neurons of the lateral hypothalamus perifornical area. No orexins found adipose tissues or visceral organs, including adrenal gland. However, expression orexin-receptor 2 (OX2R) rat gland has reported. To test effects on peripheral we investigated their catecholamine synthesis secretion pheochromocytoma cell line PC12. Orexin-A (100 nM) significantly reduced basal PACAP-induced tyrosine hydroxylase (TH) (the rate-limiting enzyme biosynthesis catecholamines) mRNA levels. also inhibited increase cAMP level, suggesting that suppressive effect TH is mediated, at least part, by cAMP/protein kinase A pathway. Furthermore, suppressed dopamine from PC12 cells. Next, examined whether orexin receptors (OX1R, OX2R) were present In glands, OX2R was as strongly expressed hypothalamus, but OX1R not detected. On other hand, neither nor binding assays showed equal to cells, existence these cells some for orexins. These results indicate suppress release synthesis, inhibitory mediated
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