MiRNA-543 promotes osteosarcoma cell proliferation and glycolysis by partially suppressing PRMT9 and stabilizing HIF-1α protein.

摘要: // Heng Zhang 1, * , Xiaofeng Guo 7, Xing Feng 2 Tingting Wang 3, 4, Zhaohua Hu 1 Xiangyong Que Qingsong Tian 5 Tianbo Zhu Guixian 6 Wei Huang Xinzhi Li Department of Orthopedics, Renhe Hospital, China Three Gorges University, Yichang, Hubei, Molecular Pharmacology, Rutgers New Brunswick, Jersey, USA 3 General Surgery, Beijing Friendship Capital Medical Beijing, 4 National Clinical Research Center Digestive Diseases, Medicine, College, The Second Hospital Affiliated to Guangzhou Guangzhou, Guangdong, 7 College Xiamen Xiamen, Fujian, These authors contributed equally this work Correspondence to: Li, email: xinzhilirenhe@163.com Feng, fengx1@cinj.rutgers.edu Keywords: osteosarcoma, miRNA-543, glycolysis, PRMT9, HIF-1α Received: April 17, 2016      Accepted: November 06, Published: 28, 2016 ABSTRACT Osteosarcoma (OS) is the most common primary bone tumor, occurring frequently in adolescents and possessing a high malignant severity. MicroRNAs play critical roles during OS development. Thus, elucidation involvement specific microRNAs development may provide novel therapeutic targets for treatment. Here, we showed that specimens from patients, levels miR-543 were significantly increased whereas PRMT9 decreased, compared paired normal tissue. Moreover, inversely correlated cell lines. Bioinformatics analyses predicted target 3'-UTR mRNA inhibit its translation, which was confirmed by luciferase-reporter assay. MiR-543 promoted proliferation vitro vivo . Mechanistically, inhibited PRMT9-enhanced oxidative phosphorylation, while depletion PRMT9-increased instability glycolysis cells. Clinically, expression negatively with tissues. Together, our data important evidence development, suggest targeting glycolytic pathway through miR-543/PRMT9/HIF-1α axis represent potential strategy eradicate