A live (delta aroA) Aeromonas salmonicida vaccine for furunculosis preferentially stimulates T-cell responses relative to B-cell responses in rainbow trout (Oncorhynchus mykiss).

摘要: We have previously described (L. M. Vaughan, P. R. Smith, and T. J. Foster, Infect. Immun. 61:2172-2181, 1993) the construction of a kanamycin-resistant aromatic-dependent mutant Aeromonas salmonicida, causative agent furunculosis, its use as live vaccine. Here we describe an unmarked aroA deletion examine nature magnitude immune responses in rainbow trout (Oncorhynchus mykiss) to this vaccine strain. Good proliferative antibody were elicited by using range doses from 2 x 10(6) 10(9) bacteria per fish, clear dose effect was apparent. A significant positive prime for lymphocyte proliferation production However, problem directly comparing killed bacterial preparations is discussed, since some replication vivo expected. Most importantly, preferentially stimulated enhanced T-cell responsiveness, evidenced significantly greater increases than B-cell proliferation, compared with respective cell populations fish given The manner which vaccines elicit strong cell-mediated relevance design are discussed.