Polyphasic study of the genetic diversity of lactobacilli associated with ‘Almagro’ eggplants spontaneous fermentation, based on combined numerical analysis of randomly amplified polymorphic DNA and pulsed‐field gel electrophoresis patterns

摘要: I. SANCHEZ, S. SESENA AND L.L. PALOP. 2004. Aims: The goal of this study was to assess the genetic diversity lactic acid bacteria (LAB) from complex natural ecosystem present in spontaneous fermentation ofAlmagroeggplants by a polyphasic approach based on molecular techniques. Methods and Results: Randomly amplified polymorphic DNA (RAPD) pulsed-field gel electrophoresis (PFGE) were applied 149 Lactobacillus isolates obtained that process. Two random primers, OPL-05 ArgDei-For, two rare-cutting enzymes, SfiI SmaI, chosen after preliminary testing basis band intensity distribution, used. RAPD PFGE generated electrophoretic patterns suitable for strain discrimination, but further discrimination achieved when combined numerical analysis results both methods previously SDS-PAGE whole cell protein analysis, carried out. findings indicated considerable degree genomic LAB microbiota studied especially plantarum isolates. In terms species assignment, allowed definite well-founded identification 98AE7% Conclusions: represented useful tool discriminate strains responsible pickle. typing regarded as most exact compromise yielding fewest contradictions available data. Significance Impact Study: Combined RAPD-PCR has not yet been employed an like found fermenting vegetables.