Strain identification of probiotic Lactobacillus casei-related isolates with randomly amplified polymorphic DNA and pulsed-field gel electrophoresis methods

作者: Denis Roy , Pierre Ward , Daniel Vincent

DOI: 10.1023/A:1008952023716

关键词: Lactobacillus rhamnosusTypingMicrobiologyLactobacillus caseiRAPDPulsed-field gel electrophoresisBiologyLactobacillusLactobacillus paracaseiGel electrophoresis

摘要: Typing of reference strains and isolates identified as Lactobacillus casei, paracasei or rhamnosus was carried out using randomly amplified polymorphic DNA (RAPD) pulsed-field gel electrophoresis (PFGE) analyses. Strains L. were mainly grouped in the same cluster those casei. The RAPD fingerprints ATCC 393 15820 differ from paracasei/casei further supporting classification these a separate group. profiling could be used for discrimination belonging to casei

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