Ontogeny of regeneration of β-cells in the neonatal rat after treatment with streptozotocin

摘要: We induced partial β-cell loss within the pancreas of neonatal rats using streptozotocin (STZ) to better characterize mechanisms leading regeneration postnatally. Rats were administered either STZ (70 mg/kg) or buffer alone on postnatal d 4, and endocrine was examined between 4 40 later. STZ-treated showed an approximately 60% existing β-cells a moderate hyperglycemia (<15 mm glucose), with levels returning near-control values after 20 d. Within preexisting islets, there increased cell proliferation in both insulin- glucagon-positive cells at 8 as well α-cell hyperplasia. These associated pancreatic content circulating glucagon. Pancreatic glucagon-like polypeptide-1 (GLP-1) compared control values, GLP-1/glucagon ratio changed favor GLP-1. Administration GLP-1 receptor antagonist, GLP-1-(9–39), resulted decreased recovery ST...