Control of lysozyme gene expression in differentiating HL-60 cells

摘要: We have investigated the control of lysozyme gene expression in HL-60 cells induced to differentiate into macrophage-like with phorbol myristate acetate (PMA). Differentiation, as evidenced by cellular adherence, and morphological changes corresponded temporally an increase nonspecific esterase activity. The concentration medium uninduced was 10 micrograms/10(7) cells, increasing a maximum 46 after 48 h incubation PMA (16 nM). At 72 decreased 16 cells. Intracellular activity remained constant throughout differentiation. If were exposed for 24 h, washed, then maintained normal medium, they differentiated normally, confirming their irreversible commitment differentiate. secretion these however, is markedly blunted suggesting that continued treatment required lysozyme. There no change rate extracellular degradation during level mRNA does not correlate directly amount secreted medium. Hybridization cell RNA chicken cDNA probe demonstrates moderate hybridization. modest (five-fold) between 0 36 exposure PMA, corresponding burst decreases which lower than original baseline when are still secreting substantial amounts These data suggest both transcriptional post-transcriptional controls operative differentiation They also imply necessary component macrophage-monocyte