Buoyant densities and hydration of nucleic acids, proteins and nucleoprotein complexes in metrizamide.

摘要: Abstract Metrizamide is an inert, non-ionic organic compound which dissolves in water to give dense solutions of relatively low viscosity. DNA, RNA, protein and unfixed nucleoprotein complexes all band isopycnically density gradients metrizamide. The buoyant densities nucleic acids are extremely metrizamide correspond those fully hydrated DNA RNA. proteins higher than the acids, but also molecules. Nucleoproteins at dependenton proportions acid complex. While formed single bands gradients, tested showed a bimodal distribution. reason for this unknown. does not inactivate since active catalase can be recovered after centrifugation equilibrium Chromatin unaffected by exposure