Graphene oxide derivatives with variable alkyl chain length and terminal functional groups as supports for stabilization of cytochrome c.
作者： Michaela Patila , Ioannis V. Pavlidis , Antonios Kouloumpis , Konstantinos Dimos , Konstantinos Spyrou
DOI: 10.1016/J.IJBIOMAC.2015.12.023
关键词:
摘要: In this study we report the ability of reduced and non-reduced graphene oxide-based nanomaterials (GONs), modified with variable alkyl chain length terminal functional groups, to act as effective scaffolds for immobilization cytochrome c (cyt c) using different procedures. The GONs/cyt conjugates are characterized by a combination techniques, namely atomic force microscopy, X-ray photoelectron FT-IR spectroscopies well thermo-gravimetric differential thermal analysis. effect structure groups surface chemistry GONs on efficiency, peroxidase activity stability cyt was investigated correlated conformational changes protein molecule upon immobilization. enhanced (up 2-fold) increased tolerance 25-fold) against denaturing agents observed immobilized c, indicates that these functionalized suitable nanoscaffolds development robust nanobiocatalysts.
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