Blocking ELISA Using Recombinant NcSRS2 Protein for Diagnosing Bovine Neosporosis
作者： Francine A. Sinnott , Leonardo G. Monte , Thais F. Collares , Bruno M. De Matos , Diene B. Pacheco
DOI: 10.1007/S00284-014-0737-Y
关键词:
摘要: Neospora caninum is the etiologic agent of neosporosis, which leads to economic impacts on cattle industry. The reference method for serodiagnosis neosporosis indirect fluorescent antibody test (IFAT). However, IFAT laborious, expensive, and not practicable in high throughput screening. In order facilitate serological diagnosis we developed a blocking enzyme-linked immunosorbent assay (b-ELISA) based NcSRS2 recombinant protein (rNcSRS2) polyclonal antibodies against rNcSRS2 (b-ELISA/rNcSRS2). Compared IFAT, b-ELISA/rNcSRS2 showed 93.7 % accuracy (98.7 sensitivity 88.7 specificity), suggesting its potential as diagnostic detect N. sera.
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