作者: A. Čobádiová , B. Víchová , V. Majláthová , K. Reiterová
DOI: 10.1016/J.VETPAR.2013.05.005
关键词: Ursus 、 Veterinary medicine 、 Zoology 、 Protozoan parasite 、 Genotyping 、 Neospora caninum 、 Gene 、 Molecular evidence 、 Ribosomal RNA 、 Biology
摘要: Neospora caninum is an important intracellular protozoan parasite with affinity to the central nervous system of many animals and a major causative agent repeated abortions in cattle. In total, 45 muscle, liver, or spleen samples brown bear sampled different locations Central Slovakia were examined by PCR. Genotyping N. was based on amplification Nc-5 gene ITS1 rRNA fragments subsequent sequencing. Presence DNA confirmed 24.4% (11/45) tested animals. The results obtained study confirm first molecular evidence European (Ursus arctos) Slovakia.