Early divergence of Th1 and Th2 transcriptomes involves a small core response and sets of transiently expressed genes.

摘要: Th cells can adopt a number of different phenotypes. We performed microarray-assisted mRNA profiling on antigen-stimulated, TCR transgenic murine splenocytes that were cultured in the presence cytokines. Transcriptome snapshots differentiating into Th1 and Th2 phenotypes obtained at various time points. Principal component analysis shows since activation skewing are largest sources variance (i.e. contributing factors) our experiments. Divergence between is established early does not increase terms differential genes from day 1 to 4 after stimulation. Notwithstanding lack further divergence lineages, we show gene expression best described by 'turnover' rather than 'core response' model, although find evidence for both. identify clusters skewed associated with persistent ('core response') late ('turnover') expression. In addition classical genes, members Batf transcription factor family differentially expressed particular helper phenotypes, suggesting an important role this Th-cell phenotype differentiation.