STUDY OF THE ENZYMATIC ISOMERIZATION OF GLUCOSE DURING REVERSE-PHASE LIQUID CHROMATOGRAPHY
作者： Xiuhong Wu , Hong Gao , Bingchang Lin
DOI: 10.1080/00986440903574826
关键词:
摘要: In this study, glucose and fructose were subjected to catalytic conversion by isomerase in an amino-phase packed column operated using a reverse-phase mobile phase that enabled simultaneous reaction separation. The was composed of acetonitrile aqueous solution. system used determine the apparent rate constant, kapp, constants stationary phases, km ks, respectively. static-state kinetic parameters Km Vm also investigated. This work has demonstrated isomerization reverse can be studied appropriate conditions for separation amino chromatography system.
sci-hub.se 参考文章(17)
G. Ganetsos, P. E. Barker, Preparative and production scale chromatography. Marcel Dekker, Inc.. ,(1992)
Abdelaziz Toumi, Sebastian Engell, Optimization-based control of a reactive simulated moving bed process for glucose isomerization Chemical Engineering Science. ,vol. 59, pp. 3777- 3792 ,(2004) , 10.1016/J.CES.2004.04.009
Eduardo A. Borges da Silva, Antonio A. Ulson de Souza, Selene Guelli U. de Souza, Alírio E. Rodrigues, Analysis of the high-fructose syrup production using reactive SMB technology Chemical Engineering Journal. ,vol. 118, pp. 167- 181 ,(2006) , 10.1016/J.CEJ.2006.02.007
Diana C.S. Azevedo, Alı́rio E. Rodrigues, Design methodology and operation of a simulated moving bed reactor for the inversion of sucrose and glucose–fructose separation Chemical Engineering Journal. ,vol. 82, pp. 95- 107 ,(2001) , 10.1016/S1385-8947(00)00359-4
Alexander H. T. Chu, Stanley H. Langer, Measurement of reaction rate constants in the liquid chromatographic reactor: mass transfer effects Analytical Chemistry. ,vol. 58, pp. 1617- 1625 ,(1986) , 10.1021/AC00121A007
A. Converti, M. Del Borghi, Kinetics of glucose isomerization to fructose by immobilized glucose isomerase in the presence of substrate protection Bioprocess Engineering. ,vol. 18, pp. 27- 33 ,(1997) , 10.1007/S004490050406
Guido Ströhlein, Florian Lode, Marco Mazzotti, Massimo Morbidelli, Design of stationary phase properties for optimal performance of reactive simulated-moving-bed chromatography Chemical Engineering Science. ,vol. 59, pp. 4951- 4956 ,(2004) , 10.1016/J.CES.2004.07.014
S. Caravieilhes, D. Schweich, C. de Bellefon, Transient operation of a catalytic liquid–liquid plug flow reactor for kinetics measurements Chemical Engineering Science. ,vol. 57, pp. 2697- 2705 ,(2002) , 10.1016/S0009-2509(02)00143-4
D.A. Luz, A.K.O. Rodrigues, F.R.C. Silva, A.E.B. Torres, C.L. Cavalcante, E.S. Brito, D.C.S. Azevedo, Adsorptive separation of fructose and glucose from an agroindustrial waste of cashew industry Bioresource Technology. ,vol. 99, pp. 2455- 2465 ,(2008) , 10.1016/J.BIORTECH.2007.04.063
O. Trapp, Unified equation for access to rate constants of first-order reactions in dynamic and on-column reaction chromatography. Analytical Chemistry. ,vol. 78, pp. 189- 198 ,(2006) , 10.1021/AC051655R