Evaluation of immunoenzymatic methods for the detection of aflatoxin M1 in ewe’s milk

作者: R. Rubio , M.I. Berruga , M. Román , A. Molina

DOI: 10.1016/J.FOODCONT.2009.01.007

关键词:

摘要: Ewe milk samples spiked with aflatoxin M1 (AFM1) at concentrations of 25, 50, 75 and 100 ng/kg were analysed by 5 different (Kits A to E) commercial Enzyme-Linked ImmunoSorbent Assay kits evaluate the mycotoxin recovery average. The least accurate results sensitivity obtained 25 concentration underestimation in all cases. When sample contamination-level was increased (50, ng/kg), four improved. Low coefficients variation (3–14%) indicate good repeatability. Acceptable mean recoveries (47–60 especially 50 (EC Maximum Level) tests except Kit A. We conclude that Kits B, C, D E are appropriate for screening AFM1 ewe’s milk, but not regulatory purposes.

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