Black rhinoceros (Diceros bicornis) and domestic horse (Equus caballus) hindgut microflora demonstrate similar fermentation responses to grape seed extract supplementation in vitro.

摘要: Summary The domestic horse is used as a nutritional model for rhinoceros maintained under human care. The validity of this browsing has been questioned due to high prevalence iron overload disorder (IOD) in captive black (Diceros bicornis), which associated with morbidity and mortality. Iron chelators, such tannins, are investigation dietary supplements ameliorate or prevent IOD prone species. Polyphenolic compounds variably affect microbial fermentation, so the first objective experiment was evaluate effects grape seed extract (GSE; concentrated source condensed tannins; CT) on hindgut fermentation. Equine nutrition knowledge assess rhinoceros; therefore, second fermentation compare responses GSE using continuous single-flow in vitro culture system. Two replicated experiments were conducted faeces inoculum sources comparing four diets increasing inclusion (0.0%, 1.3%, 2.7% 4.0% diet dry matter). Diet polyphenolic compositions determined, sodium sulphite effect neutral detergent fibre extraction CT-containing forages tested. Increasing stimulated growth proportionally increased CT concentration iron-binding capacity. Horse microflora nutrient digestibility did not differ, results supported equine an adequate rhinoceros. Interpretation these limited further research needed foregut absorption between two Supplementation up 4% unlikely adversely viability