Microtubules do not promote mitotic slippage when the spindle assembly checkpoint cannot be satisfied

摘要: When the spindle assembly checkpoint (SAC) cannot be satisfied, cells exit mitosis via mitotic slippage. In microtubule (MT) poisons, slippage requires cyclin B proteolysis, and it appears to accelerated in drug concentrations that allow some MT assembly. To determine if MTs accelerate slippage, we followed human RPE-1 exposed various poisons. At 37°C, duration of nocodazole, colcemid, or vinblastine inhibit varied from 20 30 h, revealing different poisons differentially depress destruction rate during The Eg5 inhibitors, which induce monopolar spindles without disrupting dynamics, was same as lacking MTs. Thus, presence numerous unattached kinetochores, do not Finally, compared with MTs, is over a range poison because SAC becomes satisfied on abnormal accelerated.