Batch kinetic studies on growth of salt tolerant Pseudomonas aeruginosa secreting protease in a biocalorimeter

摘要: Biocalorimetry has proved to be an efficient tool for studying the energetics involved in several biochemical reactions. In this study, biocalorimetry was employed simultaneously analyze biokinetics and bioenergetics during cultivation of a salt tolerant Pseudomonas aeruginosa production alkaline protease. Batch experiments were performed bench scale biocalorimeter protease by P. using optimized process conditions. Tessier’s double substrate growth model found provide good fit biocalorimeter, biokinetic parameters estimated. The heat flow profile resulting from metabolic activity shown accurately depict both kinetics cell production. Biokinetic bioenergetic analysis on revealed that peptone is preferentially used as its intracellular activities glycerol acts energy source metabolism.