Molecular characterization of a saposin-like protein family member isolated from bovine lymphocytes.

摘要: Abstract Human and porcine T lymphocytes natural killer (NK) cells produce antibacterial proteins that belong to the saposin-like family of (SAPLIP). The objective this study was determine if a bovine homolog SAPLIP exists in exhibit activity. Following stimulation with IL-2, bactericidal activity against Staphylococcus aureus detected some extent most major subpopulations including CD4 + , CD8 CD3 WC1 γδ lymphocytes. However, majority observed CD2 − lymphocytes, which are similar phenotypically NK cells. A partial sequence generated using low specificity primers designed from regions homology between other NK-lysin human granulysin. Enhanced expression lysin gene mRNA isolated IL-2–stimulated cDNA then used make specific for rapid amplification ends (RACE) procedure provided repeatable 5′ 3′ ends. By examining overlapping RACE procedure, full-length information obtained homologue. Conceptual translation demonstrated conserved similarities known members. Further characterization may facilitate its use protecting dairy cattle bacterial infections.