Unsterile and continuous production of polyhydroxybutyrate by Halomonas TD01.

摘要: An unsterile and continuous fermentation process was developed based on a halophilic bacterium termed Halomonas TD01 isolated from salt lake in Xinjiang, China. The strain reached 80 g/L cell dry weight containing 80% poly(3-hydroxybutyrate) (PHB) glucose medium during 56 h fed-batch process. In 14-day open process, the cells grew to an average of 40 60% PHB first fermentor with medium. Continuous pumping cultures second nitrogen-deficient diluted but allowed them maintain level between 65% 70% weight. Glucose conversions were 20% 30% above 50% one. This opens new area for reducing cost polyhydroxyalkanoates production.