In vitro Culture of Naïve Human Bone Marrow Mesenchymal Stem Cells: A Stemness Based Approach

摘要: Human bone marrow derived mesenchymal stem cells (BM-MSCs) resides in their niches close proximity to hematopoietic (HSCs). These naive MSCs have tremendous potential regenerative therapeutics, and may also be exploited by cancer infectious disease agents. Hence, it is important study the physiological pathological roles of MSC. However, our knowledge limited lack appropriate isolation vitro culture methods. Established methods use serum rich media, serial passaging for retrospective MSCs. primed not reflect true (Figure 1). Therefore, there a strong need direct stemness (self-renewal undifferentiated state) developmental ontogeny. We taken niche-based approach on better maintain vitro. In this approach, broadly divided as niche dependent (extrinsic), independent (intrinsic) modulatory (altruistic or competitive). Using we were able CD271+/CD133+ BM-MSCs two weeks. Furthermore, system helped us identify protective site Mycobacterium tuberculosis, causative organism pulmonary tuberculosis. review, discuss versus human BM with special focus how based could facilitate BM-MSCs.