Reduced oxygen tension attenuates differentiation capacity of human mesenchymal stem cells and prolongs their lifespan
作者： Christine Fehrer , Regina Brunauer , Gerhard Laschober , Hermann Unterluggauer , Stephan Reitinger
DOI: 10.1111/J.1474-9726.2007.00336.X
关键词:
摘要: … Cells were washed twice in ice-cold PBS, harvested in lysis buffer containing 50 mm … by wet electroblotting and blocked in PBS supplemented with 5% dry milk powder and 0.1% Tween-…
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