A theoretical simulation of hematopoietic stem cells during oxygen fluctuations: prediction of bone marrow responses during hemorrhagic shock.

摘要: The bone marrow (BM) responds to various diseases, including infections and hemorrhagic shock, by generating immune blood cells. These cells are derived from a finite number of lymphohematopoietic stem (LHSC) close the endosteal region BM. This study presumes that studies on LHSC involving proteomics, computational biology, genomics could be aided mathematical models. A theoretical model is developed predict responses proliferating (P) nonproliferating (N) BM during acute loss when Po2 in decreased. Hematopoietic were simulated for otherwise healthy individuals who have been subjected degrees loss, as represented 3%, 5%, 20% O2. robust hematopoietic activity area endosteum low loss. Steady-state hematopoiesis at oxygen saturation (80%) not with equations. Functional assays tested an vitro assay most primitive (modified long-term culture-initiating cell assay, LTC-IC). LTC-IC showed 1%, 3% - O2 mediate significant increases proliferation progenitors, compared 80% Thus, functional show used gain insights into biology different utility such surgical trauma discussed.