Molecular analysis of Vitex species using candidate DNA barcoding and PCR-RFLP of the matK gene for authentication of Vitex glabrata.
作者： Waranyoo Phoolcharoen , Suchada Sukrong
DOI: 10.1177/1934578X1300800130
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摘要: Several species of the Vitex genus, family Lamiaceae, are used in folk medicine for a variety remedies. V. glabrata is unique among because its main effect sexual enhancement. However, crude drugs derived from different might not be easily distinguishable, which could lead to their misidentification and misuse. Therefore, accurate authentication critical effective medicinal use. In this study, matK gene psbA-trnH intergenic spacer candidate DNA barcodes were sequenced analyzed identify five that medicinally Thailand: negundo, trifolia, rotundifolia, limonifolia, glabrata. Each region was successfully amplified leaves using single set primers, sequences determined. The size difference PCR products restriction fragment length polymorphism (PCR-RFLP) differentiate other species. These results indicate both as suggest PCR-RFLP analysis based on
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