Quantitative fetomaternal hemorrhage assessment with the use of five laboratory tests.

摘要: Summary Introduction In various countries, standard doses of anti-D IgG used for postpartum immunoprophylaxis hemolytic disease fetus and newborn (HDFN) vary from 100 μg to 300 μg. There are also different regulations concerning FMH assessment, opinions about applicable tests inconclusive. Methods Three flow cytometry (FCTs) with anti-D, anti-HbF, anti-HbF+CA antibodies, two modifications microscopic Kleihauer–Betke test (KBT) were used. Results In all artificial mixtures known concentrations, FCTs KBT counting 10 000 RBCs had similar satisfying sensitivity specificity. 2000 be disqualified because significant discrepancies between expected measured values FMH. The procedure was easier shorter than the remaining tests, but it can only assessment in RhD-negative mothers RhD-positive newborns. In one clinical sample, impossible distinguish fetal maternal F cells FC anti-HbF other useful. Conclusion In four correlation obtained results appropriate (CCC 1). Each some advantage limitation any situation. Therefore, is best have opportunity perform or three assays laboratory.